Review



5x stock elisa plate coating buffer  (Elabscience Biotechnology)


Bioz Verified Symbol Elabscience Biotechnology is a verified supplier
Bioz Manufacturer Symbol Elabscience Biotechnology manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 93
      Buy from Supplier

    Structured Review

    Elabscience Biotechnology 5x stock elisa plate coating buffer
    Schematic representation of the principle of SARS-CoV-2-RBD neutralizing <t>ELISA</t> assay2.2. Assay Optimization
    5x Stock Elisa Plate Coating Buffer, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/5x+stock+elisa+plate+coating+buffer/ELISA+Plate+Coating+Buffer/pmc09288645-235-53-59
    Average 93 stars, based on 4 article reviews
    5x stock elisa plate coating buffer - by Bioz Stars, 2026-09
    93/100 stars

    Images

    1) Product Images from "Application and Validation of SARS-CoV-2 RBD Neutralizing ELISA Assay"

    Article Title: Application and Validation of SARS-CoV-2 RBD Neutralizing ELISA Assay

    Journal: Archives of Razi Institute

    doi: 10.22092/ARI.2021.356677.1890

    Schematic representation of the principle of SARS-CoV-2-RBD neutralizing ELISA assay2.2. Assay Optimization
    Figure Legend Snippet: Schematic representation of the principle of SARS-CoV-2-RBD neutralizing ELISA assay2.2. Assay Optimization

    Techniques Used: Enzyme-linked Immunosorbent Assay

    Standard curve of anti-SARS-COV-2-RBD nAbs for the in-house developed SARS-COV-2-RBD neutralizing ELISA assay
    Figure Legend Snippet: Standard curve of anti-SARS-COV-2-RBD nAbs for the in-house developed SARS-COV-2-RBD neutralizing ELISA assay

    Techniques Used: Enzyme-linked Immunosorbent Assay

    Mean ± standard deviation of OD values of different anti-SARS-COV-2-RBD neutralizing antibody standards generated by the designed neutralizing ELISA kit
    Figure Legend Snippet: Mean ± standard deviation of OD values of different anti-SARS-COV-2-RBD neutralizing antibody standards generated by the designed neutralizing ELISA kit

    Techniques Used: Standard Deviation, Generated, Enzyme-linked Immunosorbent Assay, Concentration Assay, Negative Control, Positive Control

    Cut-off value results of the in-house neutralizing ELISA; A) Optimized ROC analysis cut-off value of positive and B) Concentration and log concentration limits for the optimal established cut-off in this assay
    Figure Legend Snippet: Cut-off value results of the in-house neutralizing ELISA; A) Optimized ROC analysis cut-off value of positive and B) Concentration and log concentration limits for the optimal established cut-off in this assay

    Techniques Used: Enzyme-linked Immunosorbent Assay, Concentration Assay

    Receiver operating characteristic curve analysis for the optimal cut-off value for detecting the positive anti-SARS-COV-2-RBD neutralizing antibodies using the in-house developed neutralizing ELISA kit
    Figure Legend Snippet: Receiver operating characteristic curve analysis for the optimal cut-off value for detecting the positive anti-SARS-COV-2-RBD neutralizing antibodies using the in-house developed neutralizing ELISA kit

    Techniques Used: Enzyme-linked Immunosorbent Assay

    Serum panels using the in-house designed SARS-COV-2-RBD neutralizing ELISA assay; A) Positive control samples for the assay validation and B) Negative control samples for the assay validation
    Figure Legend Snippet: Serum panels using the in-house designed SARS-COV-2-RBD neutralizing ELISA assay; A) Positive control samples for the assay validation and B) Negative control samples for the assay validation

    Techniques Used: Enzyme-linked Immunosorbent Assay, Positive Control, Biomarker Discovery, Negative Control, Concentration Assay

    Agreement of the results between the in-house SARS-COV-2-RBD neutralizing ELISA assay and gold standard test at the in-house established cut-off
    Figure Legend Snippet: Agreement of the results between the in-house SARS-COV-2-RBD neutralizing ELISA assay and gold standard test at the in-house established cut-off

    Techniques Used: Enzyme-linked Immunosorbent Assay

    Validation parameters of the in-house SARS-COV-2-RBD neutralizing ELISA assay
    Figure Legend Snippet: Validation parameters of the in-house SARS-COV-2-RBD neutralizing ELISA assay

    Techniques Used: Biomarker Discovery, Enzyme-linked Immunosorbent Assay

    Related Articles

    Recombinant:

    Article Title: Application and Validation of SARS-CoV-2 RBD Neutralizing ELISA Assay
    Article Snippet: .. Preparation of Working Human Angiotensin-Converting Enzyme 2 Protein Solution To immobilize the hACE2 protein at 400 ng/well in 100 μl of 1X working Coating Buffer on the microtiter plates wells, a calculated volume from Stock solution vial of Recombinant His-Tag-Human ACE2 protein in a concentration of 2.8 mg/ml (Raybiotech) was diluted immediately before usage with the prepared 1X working coating buffer from 5X stock ELISA Plate Coating Buffer (Elabscience) to a required volume at the appropriate concentration (4 ng/μl), which was determined in the initial experiments. ..

    Article Title: Application and Validation of SARS-CoV-2 RBD Neutralizing ELISA Assay
    Article Snippet: To apply 50 μL of HRP-RBD working solution to fit 10 ng/well, a calculated volume from the prepared HRP-RBD stock solution was diluted with HRP-Conjugate Diluent (Elabscience) to the required volume at the appropriate concentration (0.2 ng/μl), which was determined in the initial experiments. .. To immobilize the hACE2 protein at 400 ng/well in 100 μl of 1X working Coating Buffer on the microtiter plates wells, a calculated volume from Stock solution vial of Recombinant His-Tag-Human ACE2 protein in a concentration of 2.8 mg/ml (Raybiotech) was diluted immediately before usage with the prepared 1X working coating buffer from 5X stock ELISA Plate Coating Buffer (Elabscience) to a required volume at the appropriate concentration (4 ng/μl), which was determined in the initial experiments. ..

    Concentration Assay:

    Article Title: Application and Validation of SARS-CoV-2 RBD Neutralizing ELISA Assay
    Article Snippet: .. Preparation of Working Human Angiotensin-Converting Enzyme 2 Protein Solution To immobilize the hACE2 protein at 400 ng/well in 100 μl of 1X working Coating Buffer on the microtiter plates wells, a calculated volume from Stock solution vial of Recombinant His-Tag-Human ACE2 protein in a concentration of 2.8 mg/ml (Raybiotech) was diluted immediately before usage with the prepared 1X working coating buffer from 5X stock ELISA Plate Coating Buffer (Elabscience) to a required volume at the appropriate concentration (4 ng/μl), which was determined in the initial experiments. ..

    Article Title: Application and Validation of SARS-CoV-2 RBD Neutralizing ELISA Assay
    Article Snippet: To apply 50 μL of HRP-RBD working solution to fit 10 ng/well, a calculated volume from the prepared HRP-RBD stock solution was diluted with HRP-Conjugate Diluent (Elabscience) to the required volume at the appropriate concentration (0.2 ng/μl), which was determined in the initial experiments. .. To immobilize the hACE2 protein at 400 ng/well in 100 μl of 1X working Coating Buffer on the microtiter plates wells, a calculated volume from Stock solution vial of Recombinant His-Tag-Human ACE2 protein in a concentration of 2.8 mg/ml (Raybiotech) was diluted immediately before usage with the prepared 1X working coating buffer from 5X stock ELISA Plate Coating Buffer (Elabscience) to a required volume at the appropriate concentration (4 ng/μl), which was determined in the initial experiments. ..

    Enzyme-linked Immunosorbent Assay:

    Article Title: Application and Validation of SARS-CoV-2 RBD Neutralizing ELISA Assay
    Article Snippet: .. Preparation of Working Human Angiotensin-Converting Enzyme 2 Protein Solution To immobilize the hACE2 protein at 400 ng/well in 100 μl of 1X working Coating Buffer on the microtiter plates wells, a calculated volume from Stock solution vial of Recombinant His-Tag-Human ACE2 protein in a concentration of 2.8 mg/ml (Raybiotech) was diluted immediately before usage with the prepared 1X working coating buffer from 5X stock ELISA Plate Coating Buffer (Elabscience) to a required volume at the appropriate concentration (4 ng/μl), which was determined in the initial experiments. ..

    Article Title: Application and Validation of SARS-CoV-2 RBD Neutralizing ELISA Assay
    Article Snippet: To apply 50 μL of HRP-RBD working solution to fit 10 ng/well, a calculated volume from the prepared HRP-RBD stock solution was diluted with HRP-Conjugate Diluent (Elabscience) to the required volume at the appropriate concentration (0.2 ng/μl), which was determined in the initial experiments. .. To immobilize the hACE2 protein at 400 ng/well in 100 μl of 1X working Coating Buffer on the microtiter plates wells, a calculated volume from Stock solution vial of Recombinant His-Tag-Human ACE2 protein in a concentration of 2.8 mg/ml (Raybiotech) was diluted immediately before usage with the prepared 1X working coating buffer from 5X stock ELISA Plate Coating Buffer (Elabscience) to a required volume at the appropriate concentration (4 ng/μl), which was determined in the initial experiments. ..



    Similar Products

    5x stock elisa plate coating buffer  (Elabscience Biotechnology)
    93
    Elabscience Biotechnology 5x stock elisa plate coating buffer
    Schematic representation of the principle of SARS-CoV-2-RBD neutralizing <t>ELISA</t> assay2.2. Assay Optimization
    5x Stock Elisa Plate Coating Buffer, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/5x+stock+elisa+plate+coating+buffer/ELISA+Plate+Coating+Buffer/pmc09288645-235-53-59
    Average 93 stars, based on 1 article reviews
    5x stock elisa plate coating buffer - by Bioz Stars, 2026-09
    93/100 stars
    		  Buy from Supplier

    Image Search Results


    Schematic representation of the principle of SARS-CoV-2-RBD neutralizing ELISA assay2.2. Assay Optimization

    Journal: Archives of Razi Institute

    Article Title: Application and Validation of SARS-CoV-2 RBD Neutralizing ELISA Assay

    doi: 10.22092/ARI.2021.356677.1890

    Figure Lengend Snippet: Schematic representation of the principle of SARS-CoV-2-RBD neutralizing ELISA assay2.2. Assay Optimization

    Article Snippet: To immobilize the hACE2 protein at 400 ng/well in 100 μl of 1X working Coating Buffer on the microtiter plates wells, a calculated volume from Stock solution vial of Recombinant His-Tag-Human ACE2 protein in a concentration of 2.8 mg/ml (Raybiotech) was diluted immediately before usage with the prepared 1X working coating buffer from 5X stock ELISA Plate Coating Buffer (Elabscience) to a required volume at the appropriate concentration (4 ng/μl), which was determined in the initial experiments.

    Techniques: Enzyme-linked Immunosorbent Assay

    Standard curve of anti-SARS-COV-2-RBD nAbs for the in-house developed SARS-COV-2-RBD neutralizing ELISA assay

    Journal: Archives of Razi Institute

    Article Title: Application and Validation of SARS-CoV-2 RBD Neutralizing ELISA Assay

    doi: 10.22092/ARI.2021.356677.1890

    Figure Lengend Snippet: Standard curve of anti-SARS-COV-2-RBD nAbs for the in-house developed SARS-COV-2-RBD neutralizing ELISA assay

    Article Snippet: To immobilize the hACE2 protein at 400 ng/well in 100 μl of 1X working Coating Buffer on the microtiter plates wells, a calculated volume from Stock solution vial of Recombinant His-Tag-Human ACE2 protein in a concentration of 2.8 mg/ml (Raybiotech) was diluted immediately before usage with the prepared 1X working coating buffer from 5X stock ELISA Plate Coating Buffer (Elabscience) to a required volume at the appropriate concentration (4 ng/μl), which was determined in the initial experiments.

    Techniques: Enzyme-linked Immunosorbent Assay

    Mean ± standard deviation of OD values of different anti-SARS-COV-2-RBD neutralizing antibody standards generated by the designed neutralizing ELISA kit

    Journal: Archives of Razi Institute

    Article Title: Application and Validation of SARS-CoV-2 RBD Neutralizing ELISA Assay

    doi: 10.22092/ARI.2021.356677.1890

    Figure Lengend Snippet: Mean ± standard deviation of OD values of different anti-SARS-COV-2-RBD neutralizing antibody standards generated by the designed neutralizing ELISA kit

    Article Snippet: To immobilize the hACE2 protein at 400 ng/well in 100 μl of 1X working Coating Buffer on the microtiter plates wells, a calculated volume from Stock solution vial of Recombinant His-Tag-Human ACE2 protein in a concentration of 2.8 mg/ml (Raybiotech) was diluted immediately before usage with the prepared 1X working coating buffer from 5X stock ELISA Plate Coating Buffer (Elabscience) to a required volume at the appropriate concentration (4 ng/μl), which was determined in the initial experiments.

    Techniques: Standard Deviation, Generated, Enzyme-linked Immunosorbent Assay, Concentration Assay, Negative Control, Positive Control

    Cut-off value results of the in-house neutralizing ELISA; A) Optimized ROC analysis cut-off value of positive and B) Concentration and log concentration limits for the optimal established cut-off in this assay

    Journal: Archives of Razi Institute

    Article Title: Application and Validation of SARS-CoV-2 RBD Neutralizing ELISA Assay

    doi: 10.22092/ARI.2021.356677.1890

    Figure Lengend Snippet: Cut-off value results of the in-house neutralizing ELISA; A) Optimized ROC analysis cut-off value of positive and B) Concentration and log concentration limits for the optimal established cut-off in this assay

    Article Snippet: To immobilize the hACE2 protein at 400 ng/well in 100 μl of 1X working Coating Buffer on the microtiter plates wells, a calculated volume from Stock solution vial of Recombinant His-Tag-Human ACE2 protein in a concentration of 2.8 mg/ml (Raybiotech) was diluted immediately before usage with the prepared 1X working coating buffer from 5X stock ELISA Plate Coating Buffer (Elabscience) to a required volume at the appropriate concentration (4 ng/μl), which was determined in the initial experiments.

    Techniques: Enzyme-linked Immunosorbent Assay, Concentration Assay

    Receiver operating characteristic curve analysis for the optimal cut-off value for detecting the positive anti-SARS-COV-2-RBD neutralizing antibodies using the in-house developed neutralizing ELISA kit

    Journal: Archives of Razi Institute

    Article Title: Application and Validation of SARS-CoV-2 RBD Neutralizing ELISA Assay

    doi: 10.22092/ARI.2021.356677.1890

    Figure Lengend Snippet: Receiver operating characteristic curve analysis for the optimal cut-off value for detecting the positive anti-SARS-COV-2-RBD neutralizing antibodies using the in-house developed neutralizing ELISA kit

    Article Snippet: To immobilize the hACE2 protein at 400 ng/well in 100 μl of 1X working Coating Buffer on the microtiter plates wells, a calculated volume from Stock solution vial of Recombinant His-Tag-Human ACE2 protein in a concentration of 2.8 mg/ml (Raybiotech) was diluted immediately before usage with the prepared 1X working coating buffer from 5X stock ELISA Plate Coating Buffer (Elabscience) to a required volume at the appropriate concentration (4 ng/μl), which was determined in the initial experiments.

    Techniques: Enzyme-linked Immunosorbent Assay

    Serum panels using the in-house designed SARS-COV-2-RBD neutralizing ELISA assay; A) Positive control samples for the assay validation and B) Negative control samples for the assay validation

    Journal: Archives of Razi Institute

    Article Title: Application and Validation of SARS-CoV-2 RBD Neutralizing ELISA Assay

    doi: 10.22092/ARI.2021.356677.1890

    Figure Lengend Snippet: Serum panels using the in-house designed SARS-COV-2-RBD neutralizing ELISA assay; A) Positive control samples for the assay validation and B) Negative control samples for the assay validation

    Article Snippet: To immobilize the hACE2 protein at 400 ng/well in 100 μl of 1X working Coating Buffer on the microtiter plates wells, a calculated volume from Stock solution vial of Recombinant His-Tag-Human ACE2 protein in a concentration of 2.8 mg/ml (Raybiotech) was diluted immediately before usage with the prepared 1X working coating buffer from 5X stock ELISA Plate Coating Buffer (Elabscience) to a required volume at the appropriate concentration (4 ng/μl), which was determined in the initial experiments.

    Techniques: Enzyme-linked Immunosorbent Assay, Positive Control, Biomarker Discovery, Negative Control, Concentration Assay

    Agreement of the results between the in-house SARS-COV-2-RBD neutralizing ELISA assay and gold standard test at the in-house established cut-off

    Journal: Archives of Razi Institute

    Article Title: Application and Validation of SARS-CoV-2 RBD Neutralizing ELISA Assay

    doi: 10.22092/ARI.2021.356677.1890

    Figure Lengend Snippet: Agreement of the results between the in-house SARS-COV-2-RBD neutralizing ELISA assay and gold standard test at the in-house established cut-off

    Article Snippet: To immobilize the hACE2 protein at 400 ng/well in 100 μl of 1X working Coating Buffer on the microtiter plates wells, a calculated volume from Stock solution vial of Recombinant His-Tag-Human ACE2 protein in a concentration of 2.8 mg/ml (Raybiotech) was diluted immediately before usage with the prepared 1X working coating buffer from 5X stock ELISA Plate Coating Buffer (Elabscience) to a required volume at the appropriate concentration (4 ng/μl), which was determined in the initial experiments.

    Techniques: Enzyme-linked Immunosorbent Assay

    Validation parameters of the in-house SARS-COV-2-RBD neutralizing ELISA assay

    Journal: Archives of Razi Institute

    Article Title: Application and Validation of SARS-CoV-2 RBD Neutralizing ELISA Assay

    doi: 10.22092/ARI.2021.356677.1890

    Figure Lengend Snippet: Validation parameters of the in-house SARS-COV-2-RBD neutralizing ELISA assay

    Article Snippet: To immobilize the hACE2 protein at 400 ng/well in 100 μl of 1X working Coating Buffer on the microtiter plates wells, a calculated volume from Stock solution vial of Recombinant His-Tag-Human ACE2 protein in a concentration of 2.8 mg/ml (Raybiotech) was diluted immediately before usage with the prepared 1X working coating buffer from 5X stock ELISA Plate Coating Buffer (Elabscience) to a required volume at the appropriate concentration (4 ng/μl), which was determined in the initial experiments.

    Techniques: Biomarker Discovery, Enzyme-linked Immunosorbent Assay